Design superiority of palindromic DNA sites for site-specific recognition of proteins: tests using protein stitchery.
نویسندگان
چکیده
Using protein stitchery with appropriate attachment of cysteines linking to either C or N termini of the basic region of the v-Jun leucine zipper gene-regulatory protein, we constructed three dimers--pCC, pCN, and pNN. All three bind specifically to the appropriately rearranged DNA recognition sites for v-Jun: ATGAcgTCAT, ATGAcgATGA, and TCATcgTCAT, respectively (Kd, approximately 4 nM at 4 degrees C). Results of DNase I footprinting provide strong support for bent recognition helices in leucine zipper protein-DNA complexes. Comparison of the results for pCC and pNN with those for pCN shows the design superiority of palindromic sequences for protein recognition.
منابع مشابه
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ورودعنوان ژورنال:
- Proceedings of the National Academy of Sciences of the United States of America
دوره 90 11 شماره
صفحات -
تاریخ انتشار 1993